Dear all,

I was wondering if someone has advise for me in the following:

I am trying to extract from stomach content (degraded DNA, lots of blood/non-target tissue in the sample) with the Qiagen blood and tissue kit. To be representative of the diet composition, we decided to upscale the amount of sample we want to extract from (in comparison to what is recommended from the kit). We have our sample in 1000μl ATL buffer and add 100μl Proteinase K.

After 24 h at 56C the sample is about half digested. Can I leave it to digest longer? We are short on funding so ideally I wish to avoid to just add more Proteinase K, hence also no kit that handles larger amounts.. Will my DNA get degraded after a while, or how long is it stable for in ATL at 56C?

Thanks for your tips!