How to help ToxTrac to detect an moving animal?

Hi, I would like to use ToxTrac to help me with my hornet's videos. I did 600 experiments in an olfactometer to follow the behavior of hornets. They are pretty "big" but ToxTarc never detected...

23 August 2020 8,716 4 View

Survey template to assess added value in facility management?

The survey instrument is needed to assess how a hospital construction organisation perceives added value to the customer and users in strategically managing the build.

26 December 2018 221 3 View

Residual analysis after excat Fisher test (vs. Chi-square)?

I am working with small sample size qualitative clinical data (use attach file) In this context I have used a exact Fisher test as some expected frequency < 5. More exactly I perform a perform...

28 November 2018 9,832 2 View

Is or will ponderosa pine undergo a range contraction?

based on insect or other pest pressures, crown fires, drought, etc.

04 June 2018 2,464 2 View

Transwell Migration assay ?

Dear Colleagues I am bout to explore the extravasation of tumours by in vitro modelling using a trans well (Boyden chamber) assay; I must confess this is completely new to me In essence, we are...

07 May 2017 8,296 3 View

Standard curves in qPCR for very low copy number genes ?

Dear Colleague(s) I am dealing with a gene at the moment that exhibits very low expression and is barely detectable from neat cDNA. As such if you dilute the cDNA to construct a standard curve...

01 June 2016 4,189 7 View

REST Gene expression software: Windows compatibility issues ?

23 March 2016 6,328 6 View

Housekeeper choice for real time qPCR using human heart RNA ?

Dear Colleague(s) I am well versed with real time qPCR but have never actually worked with human heart tissue to determine relative expression of a GOI, viz cytochrome p450 2J2 variant. In...

14 January 2016 2,996 2 View

Does anyone know what the composition is of the detection reagent B in the ELISA Kit for alanine transaminase?

08 March 2015 6,423 3 View

Is anyone familiar with a validated pictorial rating scale used to assess females' muscularity dissatisfaction?

We are in the process of designing a new study and are interested in finding any measures that have some preliminary psychometric evaluation that tap into assessing young women's...

08 February 2015 255 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View