My lab has unopened DMEM and aMEM kept at 4C dating back to 2021 and 2022. I am wondering if it's still safe to use them, as there are a lot of bottles left (over two dozen). I will be the first to start in-vitro work in the lab ever since covid (the lab primarily uses murine models).
Hello Angel Cabrera Pereira,
Cell culture media should be used close to, or before the expiration date. If you want to try these media which have expired 3 and 4 years ago, you may try a small comparison trial comparing the expired media with the fresh non-expired media and compare the performance of the cells in both the media. If the cells are precious and the experiment is important, then I suggest you don't try these expired media. You will be wasting time and other resources.
Best,
Dear Angel Cabrera Pereira ,
To my knowledge Glutamine is the only substance that might age (decay). If you would add additional Glutamine it should be fine (at least for basic cell culture).
Best wishes
Soenke
give it a try, and yes supplement with glutamine ...easy check and save money (even if DMEM is not the most dcostly medium)
Thank you Didier Fesquet, Sönke Weinert and Malcolm Nobre for your reply. I will have a trial with some samples and give everyone an update when I have it done.
Hi, I'm experiencing the same issue. Could you please share an update if you've already tried it?