Hi, I am planning for an assay for which I will have to spin my samples at 110000g. We have an Beckman ultracentrifuge with a fixed angle rotor - Type 70.1Ti. On the screen of the centrifuge there is only the rpm values that can be set. While converting rcf/g to rpm, I find that it depends on the radius of the rotor as well. When I looked at the radius, I found three values: rmin, ravg, rmax. I am not sure which one to use. Since it is a fixed angle rotor, the base of the tube chamber will always have higher radius value then the middle part or the top part. However, if I want to pelle something it will always be at the very botttom of the tube (unless someone does any density gradient centrifugation, I guess). So, should I take the rmax to calculate the rpm that I need to get to the specific g value?
Any suggestion will be highly appreciated!
Hello,
The RCF itself is relative centrifugal field at maximum speed therefore simply you just check the maximum speed of rotor from the choices you have!!
Hi Sumit,
You can use the following link. It is a rotor speed calculator.
http://www.sciencegateway.org/tools/rotor.htm
Good luck
By the way what is your beckman model? Generally Beckman centrifuges can convert speeds rpm to rcf or rcf to rpm. Rcf (relative centrifugal force) shows already g because g is the unit of RCF. You just use 110.000 rcf.
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