I am curious about how the ratcheting of a DNA strand through a nanopore works in (oxford) nanopore sequencing.
I think that the enzyme motor is first attached to one end of the DNA strand to be sequenced, and this dna-motor assembly is driven to the mouth of a nanopore at which point the motor attaches itself at the mouth of the nanopore or in its vicinity-- oriented in a way that threads one unravelled DNA strand through the sensing aperture of the nanopore.
If this description is correct, I wonder how the correct orientation between nanopore and enzyme motor is achieved. Can someone explain this?
Note: My original question and description used the terms "motor protein" instead of the terms "processive enzyme" and "enzyme motor". I have changed to using the latter terms following the response of Matthias Schaefer.
Ｉ ａｍ ｎｏｔ ａｎ ｅｘｐｅｒｔ ｉｎ ｎａｎｏｐｏｒｅ ｓｅｑｕｅｎｃｉｎｇ，Ｉ ｂｅｌｉｅｖｅ ＤＮＡ ｐａｓｓｅｓ ｔｈｒｏｕｇｈ ｎａｎｏｐｏｒｅ ｄｒｉｖｉｎｇ ｂｙ ｃｕｒｒｅｎｔ， ＤＮＡ ｉｓ ｎｅｇａｔｉｖｅｌｙ ｃｈａｒｇｅｄ．
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