How did you feel when you received a notification of the acceptance of your first paper?

As a novice in the world of research with the aim to publish in a peer-reviewed high-quality journal, you do all you can to get quality work. Sometimes the feedback from reviewers can discourage...

09 September 2020 147 31 View

Creating individual landuse transition maps?

Dear Fellows. I have created land-use maps of two time periods. I have created the change map which shows all the changes from one class to the other, however, I want to create the transition...

28 August 2020 9,172 2 View

What interpretation can i get from a negative Gd anomaly?

From the plot of the data acquired from the project, there was a depletion in the concentration of Gd. What could have possibly caused this?

02 March 2018 2,283 0 View

Since bacteria constitute part of the normal flora in the intestines, how can bacteria be associated as a cause of diarrhoea?

For example, Samonella spp and , E.coli are part of the normal flora of the gastrointestinal tract. So how can the identification of these organisms in faecal samples of a dog with diarrhoea be...

28 October 2016 6,665 2 View

Since bacteria constitute part of the normal flora in the intestines, how can bacteria be associated as a cause of diarrhoea?

For example, Samonella spp and , E.coli are part of the normal flora of the gastrointestinal tract. So how can the identification of these organisms in faecal samples of a dog with diarrhoea be...

09 October 2016 7,207 2 View

How many passages can normal Skin Fibroblast cells go?

I have received normal skin fibroblast cells (HF-19 & NB1RGB) in my laboratory. I want to know the number of passages the cells can go before it looses it potency.

29 May 2013 8,302 1 View

I need assistance on aspergillus fumigatus

I am working on aspergillus fumigatus and i want my fellows to help how to isolate and characterize it from four soil samples from different areas

02 January 2012 5,723 3 View

Any Survey Instruments for "Psychological Distance" in Organization or "Self-Construal"?

01 January 1970 5,090 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View