Transfection toxicity when plated on glass coverslips?

I've been using A549s for the past couple of years to transfect in my protein of interest for imaging. Recently I've started to experience issues with transfecting cells seeded on glass...

27 January 2021 6,801 3 View

What is the difference between KPIs and Balanced Scorecard ?

Measuring business performance Organisations face the intense pressure of carrying out their processes efficiently, effectively and sustainably every day (Ghosh & Fedorowicz, 2008). Companies...

30 March 2020 1,553 4 View

AGGLOMERATIVE clustering interpretation?

Have a hierarchical agglomerative clustering analysis for samples from different years. Samples from different years are rather clustering together instead of with samples from same year. This is...

28 December 2019 1,942 1 View

Is it ethical to analyze information on social media for research purposes?

I intend to study some health posts and comments on the social media with the intention of analysis such information for research purposes. I plan to maintain anonymity of the originators of the...

21 August 2019 9,213 3 View

Has any serum protein been associated with epithelial-mesenchymal-transition or mesenchymal-epithelial transition of solid tumors?

Has any serum protein been associated with the induction of EMT/MET of solid tumor cell?

24 March 2019 9,913 4 View

Can you still run a mixed ANOVA if the homogeneity of variances were violated for one of you within-group factors?

I am currently analysing data on whether there is a difference in the level of understanding between maths and science using a sample of year 1, year 2 and year 3 students. I am also looking at...

07 March 2018 2,249 6 View

What interpretation can i get from a negative Gd anomaly?

From the plot of the data acquired from the project, there was a depletion in the concentration of Gd. What could have possibly caused this?

02 March 2018 2,283 0 View

Impact factor or works cited?

Which do you think is more important, impact factor or works cited, and why?

02 January 2018 926 9 View

Plant identification

15 May 2017 2,538 10 View

Since bacteria constitute part of the normal flora in the intestines, how can bacteria be associated as a cause of diarrhoea?

For example, Samonella spp and , E.coli are part of the normal flora of the gastrointestinal tract. So how can the identification of these organisms in faecal samples of a dog with diarrhoea be...

28 October 2016 6,665 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

Fractional impervious surface (FIS) map ?

Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance

24 February 2021 8,455 2 View

Can you mention any source which presents an updates forest-fire hazard or vulnerability map of North-East India?

There exists a confusion of generating fire-hazard maps with MODIS data as that way, we cannot distinguish between shifting agriculture and wildfires. I am looking for a source that provides me...

22 February 2021 9,616 2 View

Multinomial probabilities and confidence intervals - obtaining the CI of the reference category?

Hi, Some background to my question: I have fitted 1st order multinomial Markov models with covariates. Since all probabiilties (for each row of the transition matrix) sum to 1, I estimate only...

22 February 2021 2,541 4 View

Historical wind data/map?

Hello All, I am looking for the historical wind data/map (mainly the year 2020-2021). I would like to look at the wind value and direction for the specifics locations within the US for the...

18 February 2021 9,543 4 View

What is the detection limit of ED-XRF technique for transition metals in silicon ?

Hi everybody, please, can someone tell us if the transition metals (W, Fe, Ni, etc ...) in silicon destined for solar cell manufacturing can be detected by ED-XRF technique. Note that the...

18 February 2021 6,231 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View