Nitric Oxide (NO) is a highly reactive molecule and its half life is few seconds. In that case, is there any sophisticated protocol for the measurement of Nitric Oxide concentration in seawater?
The nitric oxide (NO) concentration can be determined in seawater by fluorometric method. This method is based on the reaction that 2,3-diaminonaphthalene (DAN) that has the ability to trap NO to yield the highly fluorescent 2,3-naphthotriazole (NAT). The optimum conditions, such as fluorometric determination conditions (λex = 383 nm, λem = 410 nm), the influence of temperature and equilibrium time on the DAN trapping NO, and the influence of DAN solution on trapping NO, can be examined.
I am planning to know the NO concentration in environmental seawater sample. Since this is a signalling molecule inside the microbes, I would like to know the concentration inside the biological samples too?
Fluorometric methods are in use by addition of 2,3-diaminonaphthalene (DAN) to the medium and measuring the reaction product - 2,3-naphthotriazole (NAT) which is highly fluorescent in nature.
The nitric oxide (NO) present in sea water determined by fluorometric method. This method is based on the reaction of 2,3-diaminonaphthalene. The optimum conditions, such as fluorometric determination conditions the influence of temperature and equilibrium time on the DAN trapping NO, and the influence of DAN solution on trapping NO, can be examined.