role of genetic markers in polyploidy
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...
01 March 2021 395 3 View
Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...
01 March 2021 9,537 2 View
I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...
01 March 2021 6,985 3 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...
01 March 2021 8,415 1 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...
27 February 2021 734 4 View
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...
29 December 2020 5,169 1 View
Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...
13 December 2020 942 3 View
Turmeric is a polyploid. Mostly sterile. However, it sets seed occassionally. I have collected seed and kept in poretrays filled with cocopeat. I have not observed any germination even after 15...
28 August 2020 8,342 4 View
In case of guava crossing of Diploid (Psidium guajava, 2n = 22) with P. Molle (2n=44). Is there any chance to get viable seed set?
16 June 2020 9,317 3 View
I have been looking for an explanation or a graph that explains what each peak represents for a polyploid individual. I understand they ar the dna content of nuclei, but, 1) do they represent...
07 November 2019 509 3 View
In most Flow Cytometry (FCM) graphs in journal articles of polyploid tissue, they show evidence of polyploidy as multiple peaked histograms. Does leaf tissue of a polyploid have 2n, 3n, 4n, and...
08 September 2019 6,256 1 View
08 September 2019 9,527 1 View
Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...
26 June 2019 9,059 2 View
05 June 2019 1,890 2 View
I'm trying to find a yeast fluorescent reporter system that will glow one color when the yeast is a-type, and a different color when the yeast in alpha-type. Icing on the cake would be a third...
01 February 2019 1,602 1 View