I am new to cell culture. I have been trying to standardize my MTT assay for two cell lines, but the seeding density is not clear and this affects reproducibility. Please advise.
Most universal approach in case of maximum cell lines is seeding 5,000 cells/well of a 96-well plate. Add your drug of interest next day and culture for required incubation time.
Best way to cunt cells is hemocytometer or use an automated cell counter whichever is available..