Deepjyoti Kumar Das

8 Questions 10 Answers 0 Followers

Questions related from Deepjyoti Kumar Das

Do you have any experience with checking expression Granzyme B levels in total human PBMCs using flow cytometry?

I am checking the expression level of Granzyme B in human total PBMCs under culture conditions. I stimulate the cells for 24h and 48h. I have used both overnight BrefaldinA and BrefaldinA added 4h...

01 October 2022 9,205 5 View

Which HEK cells are better for Adenovirus propagation and why?

I want to propagate Adenoviruses: Ad5 and Ad26 using HEK cells. Among HEK293 and HEK293T, which one is better for the purpose and why?

03 September 2021 5,950 2 View

Do you have any suggestions to synthesis of positive gold nanoparticles with streptavidin on its surface?

I want to synthesize positively charged gold nanoparticles and then surface decorate with streptavidin. The ideal size would be 20nm or less. Please help.

19 March 2020 8,063 2 View

Has anyone got experience in entrapping recombinant protein in chitosan nanoparticles ?

1) I have expressed and purified a recombinant protein. The protein has been dialysed to remove any remaining imidazole. The protein is now in milliQ water after passing through PD10 column. (The...

03 June 2017 2,603 4 View

During peptide vaccine development what type of proteins can be best candidates for peptide screening?

I need to design a peptide vaccine which act against a pathogen. This pathogen can have several type of proteins like location: secretory, membrane bound, cytosolic..or funtionally: virulence,...

29 January 2016 8,198 6 View

What may be the highest possible amount of the following flavonoids to get dissolved in DMSO?

How to dissolve Luteolin, Apigenin, Rutin Hydrate, Morin Hydrate, Kaempferol and Ellagic Acid in DMSO so that the final concentration of DMSO comes down to 0.2% at 500uM concentration of the solute?

27 January 2015 9,744 1 View

How is a grooving curve made for determining cell seeding density?

I am new to cell culture. I have been trying to standardize my MTT assay for two cell lines, but the seeding density is not clear and this affects reproducibility. Please advise.

23 December 2014 6,283 1 View

Among DMSO and MTT dissolving solution (containing HCl, isopropanol and SDS), which is better in an MTT assay?

Some studies have shown use of DMSO to dissolve the formazon crystals, while others have used MTT dissolving solution. DMSO is rapid and produces colour within seconds, while using MTT dissolving...

09 December 2014 264 5 View