How I can sperate DMAE from DMAE bitartrate salt?

More Razie Baghery's questions See All

Lactobacillus activity in sourdogh?

ِDear all, We work on breads made from frozen dough using sourdough. How can the growth conditions of lactobacillus be provided in cold temperatures?

10 October 2023 2,511 0 View

What is the effect of defatting method on the production of chickpea isolate?

Dear guys I am working on the production of chickpea protein isolate. What is the effect of defatting method on the production of chickpea isolate? Is it possible to make isolate from chickpea...

16 April 2023 4,889 3 View

Does anyone have a matlab code or sth which i could use to obtain fragility curve out of ida response in seismostruct?

hi, i'd be glad if anyone has the code that i could use it. i am performing IDA in Seismostruct and i should get the fragility curve after getting IDA curve, i heard that it can be obtained using...

13 April 2019 7,090 5 View

Does anyone know a good source for verification of nonlinear time history analysis of a RC building?

hello, I'd be glad if anyone could help me. I'm looking for a verification paper that has the most information about the properties of the materials used, the sections, the reinforcements...

09 November 2018 1,467 3 View

Which one is better for IDA? SAP2000 or SEISMOSTRUCT?

which one is better for IDA? SAP2000 or SEISMOSTRUCT. I'd thank if anyone can compare these two with aspects of ease of use, more accurate responses, etc.

01 September 2018 330 8 View

What is the effect of solvents on mechanical properties of film?

Does anyone know which of the compounds of water, ethanol, acetone, and isopropanol as solvents, provides a higher mechanical strength (higher tensile strength) in the edible film (hydrophilic and...

09 April 2018 9,152 2 View

Can anyone suggest how to measure the methanol in condensed water?

Dear All, Can anybody suggest methods to measure the methanol in condensed water?

02 October 2017 8,144 6 View

Anybody knows how to solve the error in my Siesta code simulation : "semicore states in Phosphate" ?

I want to calculate the band structure of GaP using Siesta code.

18 October 2015 5,730 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How can I select cells in prime editing without fluorescence-activated cell separation?

31 July 2024 8,551 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

Frank T. Edelmann

Dear Razie,

thanks for posting this interesting question. It is not quite clear to me what you mean by separate in this case. I assume that you want to completely liberate the free dimethylaminoethanol (DMAE) from its bitartrate salt, right? In that case please check the closely related discussion cited below in which this question has been answered

https://chemistry.stackexchange.com/questions/129334/how-can-i-remove-the-bitartrate-anion-from-an-organic-bitartrate-salt

Good luck with your work and best wishes!

Afroza Begum

If you added a stoichiometric amount of potassium bicarbonate (KHCO3, quite safe) to an aqueous solution of DMAE, you would precipitate almost all of the bitartrate, leaving the DMAE in water.

İsmail Emir Akyildiz

Ion chromatography with cation exchange mode or reverse of it to get DMAE as a flow-through.