Hi all,
I really have a problem with cationic liposome aggregation in cell cultural medium. I know that salt in the medium going to induce that kind of aggregation but how fast is that? also, how can I make my liposome stable as long as I can?
sonication does not remove the reason for agglomeration, which is screening the cationic charges of the liposomes. Therefore it cannot 'protect'.
For stability in salt medium you would need to add steric (osmotic) stabilization.
Welcome,
In my opinion You should try to protect Yours liposomes w/ nanoparticles . I do not know what type of media do You have. You should try to protect liposomes using one of the simplest method e.g sonification w/ some nanoparticles. Let me know about Your results
thanks Marcin for your kind response to my question. the medium that i am using is complete medium RPMI 1640. i usually do the sonication and size exclusion for the liposome but the aggregation for the cationic liposome is very fast like within one hour youn can see the particle
sonication does not remove the reason for agglomeration, which is screening the cationic charges of the liposomes. Therefore it cannot 'protect'.
For stability in salt medium you would need to add steric (osmotic) stabilization.
thanks alot Titus Sobisch for your response, do you think to use something like surfactant is going to be enough? or do you think i have to do something else? is there any paper that you would suggest to flow?
to a certain degree nonionic surfactant with long polar group may add stabilization, however, maybe it will affect liposome structure or induce more drastic changes like formation of mixed micelles instead of liposomes. Addition of water soluble polymer, e.g. may work without leading to marked changes of liposomes.
These are only speculations, I personally did not worked on this topic.
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