We investigate the effect of nanoparticles on the treatment of plant seeds. We use small concentrations. We did not observe any toxic effects. It is. Such research is in the literature. In your case, it is necessary to consistently increase the concentration of nanoparticles in a solution that feeds plants and monitor their development. Look at our work on what parameters it can do.
As toxicity of nanoparticles depends on their particle size, concentrations and plant species. Recently, we investigate the toxic effects of CuO-NPs on spring barley in the hydroponic system (Article Toxicity of copper oxide nanoparticles on spring barley (Hor...
). You may use an increasing dose of NPs concentration or high concentration to observe the toxic effects on plant performance.