Hi! I have been processing whole blood for several years now, using Ficoll and Dextran sedimentation. However, I am about to receive new samples from pregnant women that have cesarean delivery at night and so blood would be stored for a few hours until I can get it and process it.
Do you think this is a viable option? Or should I just stick to the patients that have cesarean delivery during the day? In case I DO decide to use them, should I store the whole blood at room temp or in the fridge?
Thanks for your answers in advance. I appreciate any comment on this subject.
Since they are short-lived, neutrophils should be used within 2-4 hours of collection. The procedure consists of layering whole blood over the density gradient medium, centrifugation, separation of neutrophil layer, and lysis of residual erythrocytes. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074468/
Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668800/
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