Guillermina Calo

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Questions related from Guillermina Calo

How fast do I have to process a whole blood sample for neutrophils isolation?

Hi! I have been processing whole blood for several years now, using Ficoll and Dextran sedimentation. However, I am about to receive new samples from pregnant women that have cesarean delivery at...

21 July 2021 6,236 3 View

Has anyone ever used Dextran for human neutrophil purification from other brand than Sigma?

I have been purifying neutrophils from peripheral human blood using sedimentation with Dextran from Leuconostoc spp, Mr 450,000-650,000. I was wondering if someone has a cheaper (and effective)...

25 June 2019 983 1 View

Is there anyone with problems isolating neutrophils from bone marrow in mice?

Hi! I am trying to isolate neutrophils from mice bone marrow, using a two layer of Histopaque 1119 and 1077. I'm doing everything as the procotol say, but can't seem to get the correct halo. This...

10 August 2015 3,240 11 View