I have been purifying neutrophils from peripheral human blood using
sedimentation with Dextran from Leuconostoc spp, Mr 450,000-650,000. I was wondering if someone has a cheaper (and effective) alternative. I found MP Biomedicals, but I'm not sure it'll work.
Any suggestion is more than welcomed!
Hi Guillermina Calo -
In our case we found the most effective method that ensures neutrophil integrity is the MACSxpress Neutrophil Isolation Kit. We did look at other alternatives that are used in literature Dextran/sodium metrizoate mix as well as the Percoll solution method which doesnt require dextran however it does affect quality of neutrophils. You will find a number of literature on these methods. If I had to suggest a choice...use the MACSxpress. Hope you are successful in your experiments!
- Badges
- Science topic