How effective is it to calculate germination percentage and vigor index of seeds/ seedlings when inoculation was done to induce disease?

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The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

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Andrew Paul McKenzie Pegman

It is effective if you have a control to compare with and proper replication :)

Abhijeet shankar Kashyap

Article Analysis of correlation between seed vigour, germination and...

This research article will help you out.

Anoop Kumar Srivastava

Such attempt would be more effective , you can work out these indices in presence versus absence of pathogen....

Aremu Mariam Bukola

Percentage germination is calculated by Number of seeds planted minus number of seeds that germinate divide by number of seeds planted multiply by one hundred

Abdenour Kheloufi

https://www.zobodat.at/pdf/PHY_44_2_0231-0244.pdf

Bozena Sera

I agree with Andrew and Anoop. The experiment can be performed including the calculation of seed germination and seedling vigor indexes, but always with control samples that contain seeds without inoculation (with replications). Then compare the obtained data set by t-test.