Mutations caused by uv radiation or mutations induced by chemical reagents - which method will give the best results in increasing the yield of enzyme in case of bacteria? In which method are the chances of revert back lower?
UV will cause T-dimers and chemicals can be nucleotide modifying agents, in either case you will get activation of specific DNA repair mechanisms, if mutation rate is too high chromosomal breakage (ssDNA nicking or dsDNA breaks) will occur at a rate higher than can be repaired by the cell. Mutagenesis experiments where you want to retain certain types of mutations might have to be performed in strains mutant for specific repair mechanisms. Also your choice of mutagen depends on what you want to investigate, so you have to be more specific. There are ways to quantify mutation rates but you have to be more specific with the purpose of your mutagenesis.
As a general rule, once can increase the overall mutation rate either by broad spectrum mutagenic chemicals or by UV and you will get similar sorts of mutations. UV will also induce deletions and other events at a frequency higher than many chemical mutagens.
Revertability of individual point mutations will be essentially the same regardless of the mechanism used to induce it.
Thanks for the reply maksym and michael sir i want to increase the production of enzyme lipase
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