To make phylogenetic tree more reliable, Construct phylogenetic tree by NJ, ML,MP these three methods and validate the conserved branch and presented in a one tree by give asteric sign. You can consider recent IJSEM paper. This way you can make your phylogenetic tree more reliable.

I absolutely agree with Georg. He is absolutely correct the other answer by arnab will not give you any idea about the robustness of tree. For using the methods suggested by Goerg you can also use PAUP although old but still works fine for small number of sequences. Using bootstrap jackknife methods will increase the robustness of the trees and will try to give you the values for each node. If you have any further doubt please ask.

Bootstrapping

Use MEGA 5.0 software. You can find both distance based and character based tree generation methods. After that you can apply bootstrap to the tree just by clicking the bootstrap option. It is easy to use and comes with many functionalities. Also use the "default parameters" for each algorithm Good luck

Thanks for the answers Farahan sir i have used MEGA 4 software for construction of tree i used the default parameters checked using bootstrap.How to interpret it sir

thanks to all the researchers for their valuable answers.

Patrice sir i am not getting whats AIC and BIC models .

Thanks patrice sir

bootstrap method