Which softwares should be used and can any one give me the detailed protocol of it.
Go to :https://www.ebi.ac.uk/Tools/msa/muscle/
Paste your sequence in the space provided .
After Performing multiple sequence alignment you'll find an option as :send to clustalw phylogeny. Once you click that and give submit you'll get a phylogenetic tree.
MEGA 6 is an easy software
1) Align your seuences
2) edit the MSA [Install Jalview (http://www.jalview.org/). You can use it for MSA, editing]
3) Use Phylip,mrbayes or above mention program for Phylogeny
1-align your sequences using:
https://www.ebi.ac.uk/Tools/msa/clustalw2/
http://www.expasy.org/genomics/sequence_alignment
or https://www.ebi.ac.uk/Tools/msa/tcoffee/
These tools perform the alignment and the construction of a phylogenetic tree (check the results)
Anyone please do suggest me the topics to carry out the projects in microbiology. Which have vast application and very much useful the society. I welcome the all sort of suggestions in this regard.
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