Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

Only one clone of purified protein is active?

Hi! I'm wondering if anyone else has had a situation where they are purifying an enzyme, and only one clone is active? The cloned gene is genetically identical to other clones, but only this one...

18 September 2020 7,984 3 View

How can an enzyme have multiple optimal pH values?

Hi! I've been studying a particular enzyme that in in vitro experiments exhibits two optimal pH's. All other parameters are the same (incubation time, substrate, temperature, salt concentration,...

16 August 2020 6,073 10 View

Best phylogenetic analysis for a small data set?

I have a small data set consisting of 16 sequences, 919bp each. I am trying to determine the phylogenetic relationship among the individuals. I am wondering if the neighbor-joining method, maximum...

15 July 2020 3,240 8 View

(Geneious software) How do you DELETE the 'bad nucleotide' symbol of "?" from genome sequence?

Hi everyone! (I'm hoping you're all safe and healthy) I could really use some help figuring out how to remove the bad nucleotide symbol "???" in my Geneious sequence. (See attached photo) Before...

18 May 2020 7,840 1 View

What are the spectroscopic technics used in biological chemistry or biology ?

I want to kno more about the technicqs people usually use to learn how it works.

21 February 2020 6,917 2 View

How do I interpret interactions with the covariate in a repeated measures ANOVA?

I am running an analysis on repeated measures perceptual data (psychology). There are three categorical factors which specify the conditions participants viewed the stimuli in (e.g mode of...

30 January 2020 2,209 3 View

Can I perform XRD analysis on polished sample?

Dear All, I have to analyze a Co based superally sample where are present two different crystal structures of Co (the cubic and the exagonal one). I'm sure of it because I have analyzed the...

14 November 2018 3,667 3 View

Is there a product that allows mammalian cell lines to be stored at ambient temperature (18-25oC) for 96 h?

Hi I'm looking for a reagent that can be used to maintain cell viability at room temperature for at least 96 h. Does anyone have a recommendation? Thanks.

02 August 2018 8,793 4 View

SPSS:What should I do when my -2 log likelihood is really high, r squared value really low and a 0.000 result for the Hosmer-Lemeshow goodness-of-fit?

I am currently doing my masters thesis which involves data analysis using SPSS, the dataset has over 135000 items and numerous variables. I performed a test of multicollinearity for my...

22 July 2018 2,214 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View