How does one assign bases to chromatogram peaks?

More Shivakumara Bheemanaik's questions See All

What software can be used for drawing ORFs on genes as arrows?

I am trying to search for a software (preferably free) that could draw the arrows that represent ORFs on genes. I have multiple contigs from different bacteria with some ORFs of interest that I...

04 May 2014 5,888 0 View

What is the most suitable protocol for annotating protein from a raw sequence to a functional protein?

I want to know the best protocol for annotating protein from a raw sequence to a functional protein. There are many bioinformatic tools to perform this, but which one is best? If you mention the...

03 April 2014 8,229 2 View

How to perform Virtual DNA sequencing?

I am trying to learn how to perform virtual DNA sequencing procedure that includes base calling, sequence assembly, mapping assembly, and contig mapping. Please suggest a simple way of performing...

31 December 2013 9,300 2 View

What is the best chromatography data analysis software?

I have to demonstrate the analysis of chromatography data to students using a software. Can somebody suggest me which is the suitable software that I can download freely and use for the...

11 December 2013 10,030 1 View

Which is the best method for understanding electrostatic interactions in biomolecular systems?

While simulating protein-ligand interactions, which is the best method for understanding electrostatic interactions?

11 December 2013 6,307 10 View

Proper choice of precipitants for protein crystallization?

I am trying to understand how to chose a precipitant for protein crystallization. What should be the basis for choosing a precipitant? The size of the protein is around 100 kDa and it exists as...

10 November 2013 2,877 3 View

Could someone suggest a good text book for teaching research methodology?

I have to teach research methodology for Master degree students. This includes review of literature, data analysis and scientific writing. Please suggest good text books to teach this seminar.

09 October 2013 6,328 8 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View