How does one assign bases to chromatogram peaks?

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What software can be used for drawing ORFs on genes as arrows?

I am trying to search for a software (preferably free) that could draw the arrows that represent ORFs on genes. I have multiple contigs from different bacteria with some ORFs of interest that I...

04 May 2014 5,790 0 View

What is the most suitable protocol for annotating protein from a raw sequence to a functional protein?

I want to know the best protocol for annotating protein from a raw sequence to a functional protein. There are many bioinformatic tools to perform this, but which one is best? If you mention the...

03 April 2014 8,127 2 View

How to perform Virtual DNA sequencing?

I am trying to learn how to perform virtual DNA sequencing procedure that includes base calling, sequence assembly, mapping assembly, and contig mapping. Please suggest a simple way of performing...

31 December 2013 9,202 2 View

What is the best chromatography data analysis software?

I have to demonstrate the analysis of chromatography data to students using a software. Can somebody suggest me which is the suitable software that I can download freely and use for the...

11 December 2013 9,920 1 View

Which is the best method for understanding electrostatic interactions in biomolecular systems?

While simulating protein-ligand interactions, which is the best method for understanding electrostatic interactions?

11 December 2013 6,168 10 View

Proper choice of precipitants for protein crystallization?

I am trying to understand how to chose a precipitant for protein crystallization. What should be the basis for choosing a precipitant? The size of the protein is around 100 kDa and it exists as...

10 November 2013 2,773 3 View

Could someone suggest a good text book for teaching research methodology?

I have to teach research methodology for Master degree students. This includes review of literature, data analysis and scientific writing. Please suggest good text books to teach this seminar.

09 October 2013 6,234 8 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View