Being a toxic metabolite, MG is detoxified in the cell. However, certain studies show that they have treated cells (yeast) with mM amounts of MG. So how is it getting inside the cell?
I have been purifying His-tagged protein using Ni-NTA beads. I have been successful in eluting the wild type protein. Elution buffer constituents are: Tris 50mM, KCl 0.5M, Imidazole 500mM....
29 August 2023 3,132 3 View
My EMSA results show smears of DNA protein complex instead of band, maybe because of non stable protein DNA complex. I tried reducing salt concentrations, but it didnt work. I have also tried...
30 January 2023 9,162 6 View
I want to add Land cover categories from the lookup table. I got an error message describing "Argument length must be greater or equal to zero". How can I resolve this issue?
17 December 2018 5,037 6 View
I want to measure the amount of FADH2 produced in different variants of yeast cells. There are kits available for the measurement of FAD by sigma.
02 December 2018 4,593 1 View
Hello Everyone, I am trying to analyse cytokine production from lung cells and splenocytes from infected and uninfected (naive) mice for FACS analysis. As of now, I tested splenocytes and lung...
03 August 2017 3,160 4 View
I would like to try and stabilize a protein that is tagged with FLAG tag extracellularly with the help of anti-FLAG anitbody. Such experiments have been done in cell culture, but I have to do it...
06 September 2015 1,336 1 View
Any recorder that comes with a good software for transcribing?
07 October 2013 891 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View
We have been using MFC to control the flow rate of pure ammonia gas. The MFC is placed in an oven at 50 deg. C to avoid blockage due to NH3. However, after a few days of usage, the MFC is unable...
11 July 2024 5,796 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
In most colorimetric determinations of methylglyoxal, there is neutralization using saturated potassium carbonate, and perchloric acid is added to the final reaction. I would like to understand...
23 June 2024 2,074 0 View
Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?
05 June 2024 6,447 1 View
Comprehensive Review of Warfarin How does warfarin toxicity present clinically, and what are the recommended management strategies for patients experiencing warfarin toxicity?
05 June 2024 5,100 1 View
*Natural products such as berberine or curcumin.
26 May 2024 3,367 3 View
Currently, there is a significant issue of iron deficiency among many people, leading to widespread use of iron supplements. However, many are unaware that iron can be toxic, and excessive iron...
24 May 2024 6,865 1 View
I need the reason why stored food crops become toxic to living organisms when eaten.
22 April 2024 6,900 1 View