I know if we increase the mg ion concentration and if we supplement the manganese the fidelity of the Polymerase is reduced. Why manganese is essential for low fidelity? what is the role of manganese on the fidelity of polymerases?
The mechanism is related to Mn2+ being a poor substitute for the required Mg2+. See
https://www.jstage.jst.go.jp/article/bpb/30/4/30_4_842/_article
and refs therein
thank you!
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