Billie Jean

7 Questions 5 Answers 0 Followers

Questions related from Billie Jean

My NEB HiFi DNA Assembly reaction is not working, I cannot ligate the plasmid. Could you help me?

Details of the experiment: We aim to clone 3 gene segments into a vector. Details of the gene segments: The first segment has a concentration of 34 ng/µL and is 1445 base pairs long. The second...

09 September 2024 5,835 3 View

Why does HiFi DNA Assembly (Gibson) not working in my experiments?

I am trying to add 3 fragments to a vector. All 3 fragments are different sizes (125 bp, 4578 bp, 1445 bp). But I can't reach the correctly combined plasmid. I use 1 uL of each fragment and...

21 August 2024 3,014 1 View

What is the Best Midiprep Plasmid Isolation Kit?

Hello, I want to order Midiprep plasmid isolation kit, but I can't decide which brand to use. Can you recommend the brands you always use and think are highly effective? How about GeneJet? Thank You.

01 July 2024 5,944 2 View

From which protocol can I obtain a high number of bacterial cell?

I want to precipitate my S.aureus bacteria cells but I couldn't find a reliable protocol to precipitate, obtain high number of bacteria. Can you give me a protocol to precipitate bacterial cells?

29 December 2022 4,088 6 View

How does manganese reduce the fidelity of DNA polymerases?

I know if we increase the mg ion concentration and if we supplement the manganese the fidelity of the Polymerase is reduced. Why manganese is essential for low fidelity? what is the role of...

10 October 2022 4,041 2 View

Best transformation type for bacteria

According to what should I choose the best plasmid transformation type?

01 January 1970 2,626 1 View

Nanodrop results of digested plasmid

Hi, I ran the digested plasmid on gel and purified it separately with NEB and QIAgen gel extraction kits, but the results came like this, how should I interpret these results? Where am I going...

01 January 1970 9,933 3 View