How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 4,865 3 View

Are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

12 October 2020 8,924 1 View

Simulation Parameters for p-type and n-type layers of a typical silicon soalr cell?

Hi, I would like to simulate a typical polycristalline silicon cell with p-type and n-type layers using a drift diffusion model. I have found a lot of references for intrinsic silicon parameter...

03 February 2020 3,203 6 View

Intrinsic tryptophan fluorescence of protein measured FI using a fluorescence microplater reader based on filter or monochromator?

I was wondering if microplate reader recording filter based flourescence emission spectrum are good to measure protein protein Intrinsic tryptophan fluorescence? How does it compare with...

13 January 2020 6,354 2 View

Can I use a monthly average solar spectrum to represent the spectrum of the month?

I would like to calculate the monthly average of the representative solar spectrum for each month based on minute spectral irradiance measurements. Can I consider this hypothesis? Has anyone made...

02 October 2019 7,380 4 View

Method for Identification of Bacterial Natural products by HPLC and LC-MS-MS using databases?

I would be grateful if you could please indicate public libraries used for the identification of natural products produced by bacterial using LC/MS/MS data and/or HPLC DAD analysis

16 September 2019 7,077 4 View

When you recommend to cut the heart in a murine models of heart failure?

Good evening everybody, I am working on a model of acute heart failure and I am planning to analyse heat shock proteins (western blot) in the right and the left ventricle. The mouse heart is very...

03 June 2019 7,528 5 View

Do you know successful Agriculture and Rural Credit Guarantee schemes?

Agriculture and Rural Credit Guarantee Schemes face additional challenges if compared with urban SME's Guarantee Schemes, especially related to climate, pricing and insurance issues. I would be...

17 March 2019 2,863 4 View

Can I substitute Qiagen Buffer QG (from dna extraction kit) for Buffer ERC (Minelute reaction cleanup)?

I want to perform clean up on my digests but currently out of ERC from minElute clean up kit. Can I substitute it with buffer QG ? These are all from qiagen. Somebody told me I could do this but...

04 October 2017 8,150 3 View

Thoughts on performing PCR on purified, digested dna that has already been amplified (PCR) prior to digestion?

02 October 2017 3,916 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View