Luciano Huergo

8 Questions 7 Answers 0 Followers

Questions related from Luciano Huergo

Covalent coupling IgG to HPR using HPR glycan and IgG primary amine?

I am looking for detailed and simple protocol to couple IgG with HPR using HPR glycans and IgG amine using periodate reaction. Best if no dyalisis is required. I plann to use antibodies attached...

08 August 2020 9,469 0 View

Does anybody know the stability of SARS-CoV-2 N protein structure in response to heat (Tm) and Tween 80 0.1%?

Does anybody know the stability of SARS-CoV-2 N protein structure in response to heat (Tm) and Tween 80 0.1%? Or even heat and tween combined?

29 June 2020 2,875 4 View

How does Chai qPCR system perfom for protein melting?

Has anyone used chai qPCR machine for protein melting analysis (protein thermal shift analysis) using sypro orange? Also, how is the software helpful for that ? How good is the tecnhical support?...

10 April 2020 2,227 0 View

How to run multi omics analysis in XCMS?

I am runing pair and multi metabolome analysis in XCMS and I want to load proteomics data to run multi omics anlysis. When the metabolomic analysis is finished I enter Uniprot protein codes in...

23 January 2020 2,474 1 View

Intrinsic tryptophan fluorescence of protein measured FI using a fluorescence microplater reader based on filter or monochromator?

I was wondering if microplate reader recording filter based flourescence emission spectrum are good to measure protein protein Intrinsic tryptophan fluorescence? How does it compare with...

14 January 2020 6,462 2 View

Method for Identification of Bacterial Natural products by HPLC and LC-MS-MS using databases?

I would be grateful if you could please indicate public libraries used for the identification of natural products produced by bacterial using LC/MS/MS data and/or HPLC DAD analysis

17 September 2019 7,185 4 View

Is his drop out supplemented really needed for bacterial two hydrid bacterial match II?

The stratagene bacterial match II two hydrid system uses M9 ammonium as base media for growth. The recipient strain has a hisB mutation which is complemented with yeast his3 gene upon...

19 August 2016 3,091 3 View

Voluteer help for researchers working with SARS-CoV2

I am offering help for those researchers working with SARS-Cov2. I do have experimence in general molecular biology, sequence analysis, protein structure analysis and bionformatics. Please send...

01 January 1970 7,167 3 View