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Questions related from Luciano Huergo
I am looking for detailed and simple protocol to couple IgG with HPR using HPR glycans and IgG amine using periodate reaction. Best if no dyalisis is required. I plann to use antibodies attached...
08 August 2020 9,541 0 View
Does anybody know the stability of SARS-CoV-2 N protein structure in response to heat (Tm) and Tween 80 0.1%? Or even heat and tween combined?
29 June 2020 2,943 4 View
Has anyone used chai qPCR machine for protein melting analysis (protein thermal shift analysis) using sypro orange? Also, how is the software helpful for that ? How good is the tecnhical support?...
10 April 2020 2,272 0 View
I am runing pair and multi metabolome analysis in XCMS and I want to load proteomics data to run multi omics anlysis. When the metabolomic analysis is finished I enter Uniprot protein codes in...
23 January 2020 2,552 1 View
We are doing metabolomic analysis in Bacteria comparing diferent cultures, strains and so on. Cells are always collected at same OD by centrifugation and metabolites are extracted from cell...
22 January 2020 4,314 3 View
I was wondering if microplate reader recording filter based flourescence emission spectrum are good to measure protein protein Intrinsic tryptophan fluorescence? How does it compare with...
14 January 2020 6,524 2 View
I would be grateful if you could please indicate public libraries used for the identification of natural products produced by bacterial using LC/MS/MS data and/or HPLC DAD analysis
17 September 2019 7,258 4 View
The stratagene bacterial match II two hydrid system uses M9 ammonium as base media for growth. The recipient strain has a hisB mutation which is complemented with yeast his3 gene upon...
19 August 2016 3,137 3 View
I am offering help for those researchers working with SARS-Cov2. I do have experimence in general molecular biology, sequence analysis, protein structure analysis and bionformatics. Please send...
01 January 1970 7,232 3 View