PCR inhibitor: Isopropyl acetate (2.5%)?

If extracted viral RNA contains 2.5% isopropyl acetate, would it be acting as PCR inhibitor?

20 October 2020 3,058 3 View

Does all mineral oil contains asphaltenes?

I had a mineral oil of 460cSt (containing sulphur and phosphorus), after addition of nanparticles (carbon nano tubes and graphene) the viscosity decreased significantly. literatures says that the...

16 August 2018 6,476 1 View

Which is more preferable mixed lubrication regime or boundary lubrication regime?

04 July 2016 1,862 6 View

How does Young's modulus,shear modulus,Poisson ratio of a nano wire/tube/particle influence the coefficient of friction?

Mechanical properties influencing coefficnet of friction.

21 June 2016 1,344 2 View

Can we get lower coefficient of friction with larger scar diameter in a Four Ball Test rig?

Two tests were carried out. One with a vegetable oil and the other with mineral oil. The mineral oil viscosity was almost twice than the vegetable oil. It was observed that the Coefficient of...

10 January 2016 8,250 6 View

I performed an experiment and the following were negative outputs in the response table. Why?

An L9 orthogonal DOE was performed using Minitab. The S/N in case of frictional force is negative.The response of the frictional force and rpm were negative. But in case of other output...

10 July 2015 6,731 5 View

While analyzing a pin-on-disc experiment using Taguchi analysis which type of problem has to be considered- static or dynamic?

21 March 2015 3,441 2 View

In pin on disk how are you calculating the total wear track distance?

Formula.

12 May 2014 4,071 10 View

When we add solid particles to a liquid what can we call them: emulsions or dispersions?

Addition of additives in lubricant is to be called what?

06 May 2014 6,107 9 View

What is the main reason that nano particles get agglomerated?

Nano particles such as copper oxide, zinc oxide and carbon nano tubes.

23 March 2014 9,249 40 View

Which is an appropriate Papillary Thyroid cancer cell line with the BRAF V600E mutation to work on?

Papillary thyroid cancer is a common endocrine malignancy, and the BRAF V600E mutation confers it with aggressiveness and metastatic potential. I need a proper cell line, to study this form of cancer.

02 March 2019 5,301 1 View

Gene sequencing to check a SNP?

Hello, I have two cells lines: A375 and Mel-ST. This last one is an immortalized cell line from primary melanocytes. The BRAF gene should be mutated with V600E mutation in A375 cells but not in...

21 June 2018 8,561 6 View

Gene sequencing to check a SNP?

Hello, I have two cells lines: A375 and Mel-ST. This last one is an immortalized cell line from primary melanocytes. The BRAF gene should be mutated with V600E mutation in A375 cells but not in...

05 June 2018 2,538 7 View

MD simulation of protein-ligand complex using the Gromacs program available at The Bioinformatics Resources & Applications Facility (BRAF) at C-DAC?

I have taken The Bioinformatics Resources & Applications Facility (BRAF) at C-DAC. But donot know how to use it for MD simulation of protein-ligand complex using Gromacs program. Could...

04 May 2018 212 6 View

MD simulation of protein-ligand complex using the Gromacs program available at The Bioinformatics Resources & Applications Facility (BRAF) at C-DAC?

I have taken The Bioinformatics Resources & Applications Facility (BRAF) at C-DAC. But donot know how to use it for MD simulation of protein-ligand complex using Gromacs program. Could...

02 May 2018 2,401 4 View

How to optimize allele specific PCR for detection of BRAF muatation ?

When OF(1ul) and outer OR(1ul) primers and IF(0.5 ul) and OR( 0.5 ul ) are used , it gave faint control band .And when only OF and OR primers are used it gave bright control band .How can it be...

11 April 2018 5,594 3 View

Has anyone made a BRAF resistant melanoma cell line?

I was wondering if anyone has any experience with making a BRAF resistant melanoma cell line. Preferably Dabrafenib or Vemurafenib resistance. If so, how many passages did i take and what cell...

03 April 2018 5,951 4 View

How to do simulation on BRAF server??

How can I transfer my files from my university server to BRAf server to perform MD simulation and how can I use environment module ??

03 April 2018 8,735 1 View

How to optimize allele specific PCR for detection of BRAF muatation ?

When OF(1ul) and outer OR(1ul) primers and IF(0.5 ul) and OR( 0.5 ul ) are used , it gave faint control band .And when only OF and OR primers are used it gave bright control band .How can it be...

03 April 2018 6,145 3 View

Immunohistochemistry of skin tumors in mice?

Hello everyone! I am going to perform immunohistochemistry on skin sections from mice. I am looking for a good antibody to look at pMEK and to look at BRAF(V600E) in mouse. Do you have any advice?...

08 September 2017 9,926 0 View