Papillary thyroid cancer is a common endocrine malignancy, and the BRAF V600E mutation confers it with aggressiveness and metastatic potential. I need a proper cell line, to study this form of cancer.
I am just adding in the Cellosaurus the very detailed info in the very recent review paper:
"Comprehensive Genetic Characterization of Human Thyroid Cancer Cell Lines: A Validated Panel for Preclinical Studies"
DOI=10.1158/1078-0432.CCR-18-2953
and in term of PTC with a BRAF V600E I noted:
CUTC5:
https://web.expasy.org/cellosaurus/CVCL_W916
CUTC60:
https://web.expasy.org/cellosaurus/CVCL_VM61
Both cell lines have just been published in:
https://www.ncbi.nlm.nih.gov/pubmed/30733375
IHH-4:
https://web.expasy.org/cellosaurus/CVCL_2960
Available from JCRB
K1:
https://web.expasy.org/cellosaurus/CVCL_2537
Which is in fact GLAG-66 but which does not seem to be distributed.
LAM1:
https://web.expasy.org/cellosaurus/CVCL_W912
LAM136:
https://web.expasy.org/cellosaurus/CVCL_RP38
MDA-T120:
https://web.expasy.org/cellosaurus/CVCL_QW85
MDA-T32
https://web.expasy.org/cellosaurus/CVCL_W913
MDA-T41
https://web.expasy.org/cellosaurus/CVCL_W914
MDA-T85
https://web.expasy.org/cellosaurus/CVCL_QW84
All four MDA-Txxx cell lines are distributed by ATCC
Best regards
Amos Bairoch
My current research is on Papillary thyroid cancer cell lines. For which I have purchased the cell line MDA T32 from ATCC. I need an exact description of the percentage of FBS and pen/strep to be...
08 September 2019 885 4 View
I have isolated cortical neurons from mouse pups ( day1) and seeded on poly d lysine coated wells of 24 well plated (120000cells/well) and cultured in a medium composed of Neurobasal A, Glutamax,...
05 June 2019 9,982 2 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
02 March 2021 3,060 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View