Agar plates amended with substrates (eg, CMC, casein) can be used to screen for bacteria producing active enzymes such as proteinases and cellulases. Similarity, inhibition zone in Burkholder agar assay is used to indicated antibiotic activity of bacteria isolates. I know that is a semi-quantitative method.  My question is when we measure the halo size, what data should you use: ratio (halo : colony), difference (halo - colony), or just halo size? and Why?  How halo size relate to activity? Is the relationship linear, log or something else? 

More Yijie Daniel Deng's questions See All
Similar questions and discussions