Angel Josue Arteaga

7 Questions 13 Answers 0 Followers

Questions related from Angel Josue Arteaga

Which species commonly grow on PALCAM and OXFORD Agar in addition to Listeria spp. and Bacillus?

I'm working analyzing new treatments to eliminate pathogen flora in food such as Listeria, but we have a problem: We use a strain control of Listeria innocua but we found other bacterial species...

15 May 2015 3,057 7 View

What is the protocol for a fungal sample for SEM?

I'm working with Cladosporium cladosporioides, and I need to do an analysis by SEM to see the morphology characteristics and interaction with linocellulosic substrate. Anyone have a protocol for...

04 November 2013 3,234 2 View

Can someone advise on the best way to remove a dye from a crude extract of enzymes?

I have a crude extract of enzymes produced by Solid State Fermentation, but the microorganism produces a black/ brown dye. I already tried to different methods to remove dye from extracts, like...

05 July 2013 4,735 2 View

Does anyone have experience with cellulase purification by size exclusion Chromatography (Gel chromatography)?

I'm trying to separate cellulases from a crude extract, obtained from a Solid State fermentation of a Filamentous fungi with lignocellulosic waste. In a Zymogram we confirm the Molecular weight of...

04 July 2013 1,883 2 View

Does anyone have experience with molecular characterization of a filamentous fungi?

We isolated one fungi from the soil, and from its morphology we concluded it must be a Cladosporium genera ( a filamentous fungi , a mold ). I've revised different methods, but I dont know what...

01 July 2013 9,701 9 View

What is the difference between use Sintetic substrates and natural substrates to evaluate the enzymatic activity?

In the determination of b-Glucosidase activity we used different substrates: the natural substrate cellobiose and a sintetic substrate pNGP (p-nitrohpenyl B-D glucopyranoside). In the method with...

25 May 2013 808 0 View

Enzyme Precipitation - Ammonium Sulfate or Acetone?

I am working in the production of cellulase from fungus (molds) like Aspergillus niger, but I have problems with the precipitation with ammonium sulfate. I don't see any precipitation in the...

07 April 2013 4,616 18 View