I need to establish this in my lab and I need to figure out how to do it. Also, apart from TEER measurement, are there any other ways to measure intestinal permeability in vitro?
For ex-vivo measurement if TER you can utilize modified or original Ussing chambers. You can also measure permeability of fluorescent or radio-labeled molecules with this method as well. For in vitro studies you can still measure TER using Ussing chambers or electrode tongs with trans-well plates, as well as permeability of labeled markers. You can also do immunohistochemistry to look at tight junction proteins or other markers of permeability. Most measures outside of chambers involve blood makers but some may be doable in tissue/cells as well.
Ussing Chamber for tissue; See "A guide to Ussing chamber studies of mouse intestine" by Lane Clarke (http://ajpgi.physiology.org/content/296/6/G1151).
Very insightful and a very good paper. You will need the Ussing Chamber equipment and amplifier etc. Not cheap but they are fantastic... this is for animal tissue... otherwise if you use cell monolayers, just a very sensitive multi-meter/amplifier would suffice... but it needs to be able to measure resistance (Ohms) and current/voltage...
simply all TEER measuremetns are based of Ohms law (I=V/R) Thus R=V/I; hence a change in resistance (TEER; or R in Ohms law) will result in a change in current if your voltage is clamped.. this can me measured in the ussing chamber with the correct amplifier equipment.
Hello, Sridhar. I know this thread is a little old, but I thought it would be helpful for anybody that comes across this topic to know about the Flocel line of 3D cell culture systems! Our company develops 3D cell barrier culture systems for in vitro study of virtually any cell barrier, and the system comes equipped with both a Fluid Perfusion Unit and a Trans-Endothelial/Epithelial Electrical Resistance (TEER) Measurement Unit! The use of a vessel-shaped 3D environment has been proven more effective than 2D systems like Transwell (Article Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L...
), and our advanced TEER Measurement Unit allows for frequency sweeps between 0.1-1,000 Hz, automated time point sampling, logging of data to Excel, and additional measurement of phase angle for cell capacitance calculations! For those that are committed to Transwell use, we also have a TEER Measurement Unit that is compatible with nearly all Transwell products (Endohm cell cup chamber, STX2 "chopstick" electrodes, etc) and allows the user to greatly expand their testing capabilities with Transwell equipment. Additionally, we have smaller modular systems that connect to microscope-friendly cell culture modules: i.e., a miniaturized 3D cell culture system that you can use right under your microscope! If you or anybody on this thread is interested in learning more, I encourage you to visit www.flocel.com or email me at [email protected], thank you!
For initial tests (or if you have to deal with limited financial ressources) it might be interesting to build your own TEER measurement device as shown here:
Theile M, et al. A simple approach to perform TEER measurement using a self-made volt-amperemeter with programmable output frequency. J. Vis. Exp., 2019, https://www.jove.com/video/60087
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