Sridhar Radhakrishnan

6 Questions 1 Answers 0 Followers

Questions related from Sridhar Radhakrishnan

How do you measure transepithelial electrical resistance in intestinal epithelial cells and what equipment is needed? Could anyone share a protocol?

I need to establish this in my lab and I need to figure out how to do it. Also, apart from TEER measurement, are there any other ways to measure intestinal permeability in vitro?

12 November 2014 5,568 4 View

Where to purchase a cellometer?

Our lab is planning to purchase a cell counter for routine cell culture application, and we would appreciate any suggestions where to buy it.

16 November 2013 3,759 3 View

Seeking help with transfection problems

I am trying to transfect Caco-2 cells and THP-1 (differentiated to macrophages) using Fugene HD transfection reagent. Plasmid is PGL4 NFKB luciferase (firefly). I have tried transfecting in 12, 24...

01 August 2013 7,500 12 View

I want to measure lipid profile (TC,TG,HDL,LDL,VLDL) of porcine serum sample. Is there a commercially available kit to do this or any chemical method?

I do not have a strong background in this area. Most publications use some kind of autoanalyser, but I am not familiar with this.

04 January 2013 2,934 10 View

Is there any special protocol for RNA extraction from adipose tissue?

I use a commercial kit for RNA extraction, however I am not sure if it is suitable for adipose tissue. However, due to a high content of triglycerides do I need to have an extra step or something...

15 December 2012 6,618 6 View

DNAse treatment in RNA extraction - Kunitz?

I am trying to use an optional DNA treatment from Amresco company, check attached documents for product details. I am not quite familiar with DNAse units. So I am confused. The product I bought...

05 December 2012 600 4 View