I am getting sparkling spots in the gel images after the gel documentation so that I am not getting a clear gel image.Please suggest solution to get rid of this problem.
Is is advisable to do reliability test (e.g. Cronbach Alpha Test) for a whole data set? Or is it okay to do it for a segment of data with the same characteristics.
26 May 2024 3,594 1 View
to compare against the 8H standard from regulator, How do i workout averaging for any particular date and fetch the 8 H average value and compare for the compliance/ exceedance...is it rolling...
30 March 2024 4,411 2 View
Hello everyone, I am trying to run Rietveld refinement on my sample which has two phases. The primary phase is quite dominant with most of the peaks, but there is only one peak of the secondary...
04 March 2024 9,831 2 View
is there any protocol to be followed to use ChatGpt for research?
05 January 2024 9,878 1 View
I'm having trouble locating a list of Scopus indexed journals that are freely accessible. Could you assist me with this matter?
22 November 2023 8,436 3 View
I'm in search of the 143-item version of the Temperament Evaluation of Memphis, Pisa, Paris, and San Diego Autoquestionnaire (TEMPS-A). More importantly, I'm looking for the scoring instructions...
16 October 2023 1,858 0 View
Dear All, I am trying to run the Rietveld refinement using Profex application. I am facing issues with the refinement as I am unable to bring down my Chi2 value below 2.20. I watched all the...
03 July 2023 5,054 2 View
I need, how many elements are present in the sample and how to find out from the sample.
17 April 2023 6,895 4 View
I am currently undergoing a research on: The Spatial Analysis of the Incidence and Users' Fear of Crime at Motor Parks in Ibadan. What is/are the best method for determining the sampling frame or...
29 January 2023 2,224 2 View
Should I choose the immobilization method based on the application which I am supposed to do.
10 January 2023 9,110 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,478 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...
05 August 2024 8,025 7 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,672 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,404 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 949 4 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,662 6 View