I have attempted vortexing in 200uL of 0.9% saline, but I found that the saline pH varied unless it was being spun. Because of the small volume of cecum collected I cannot dilute to a large enough volume that can be spun, so I am currently diluting in elga water thinking that this will have a more steady pH. How do you measure the pH, which solutions do you use, what volume do you mix the cecum in?
Thanks!
Dear Sir. Concerning your issue about the measurement of the pH of cecal content . The pH of the rat intestinal tract was decreased by the presence of a microbial flora, but its influence in the forestomach is less clear. Stomach pH values varied according to the amount of food present at the time of measurement. Fasting increased the pH of the gastrointestinal tract in conventional rats but had little effect in germfree rats. In the conventional rat, feeding a purified diet compared with a commercial diet resulted in a lower pH in the forestomach and a higher pH in the caecal contents. Magnesium trisilicate promoted gastric emptying in conventional rats and its antacid effect was observed only in the caecum and colon. I think the following below links may help you in your analysis:
http://journals.sagepub.com/doi/pdf/10.1258/002367787781268693 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009533/pdf/WJG-20-5000.pdf
https://www.professionalanimalscientist.org/article/S1080-7446(15)30179-0/pdf
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