We have active antibacterial compounds and they are highly lipophilic. So we are using 10% kolliphor and 10% DMSO to solubilize them in micelles. When we test this formulation for MIC we don't see any activity. We have tried shaking the plates at 250rpm while incubating the mic plates and adding tween20 up to 30% to disrupt the micelles but have not been successful.