I am doing RNA isolation using QIAzol lysis reagent. After collection of upper aqueous phase, it says add 1.5 volumes of 100% ethanol? how much do I need to add and how to prepare?
'Add 1.5 volumes' means that whatever the volume of your collected aqueous phase is, you add 1.5 times that much. So if your aqueous phase is 400uL, you would add 600uL of 100% ethanol.
Hello Shakshi Sharma
You need not prepare but just add. Suppose you have collected X ul of the upper aqueous phase, then just add 1.5 x X ul of 100% ethanol.
Just take -approximately- the volume of your aqueous phase and multiply by 1.5.
So if your aqueous phase is 600uL, you would add 900uL of 100% ethanol.
Malcolm Nobre Jose gabriel Vergara Meza Laura Leighton
Thank you so much you all
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