Olga Soto

4 Questions 5 Answers 0 Followers

Questions related from Olga Soto

Does anybody have a successful protocol or tips to Co-IP Androgen receptor with other proteins?

I have been trying to Co-IP AR with a Peroxiredoxin unsuccessfully. I am working with a knockout cell line for negative control. Co-IP conditions are as follows: Lysis buffer: 50mM TrisHCl pH...

02 February 2018 560 2 View

Native gel for protein of pI 8.83-8.89?

I am currently working on running a native gel for my chemokine receptor which has a pI between 8 and 9. It is approximately 42kD on SDS-PAGE, but due to issues in visualizing any bands, I am now...

02 February 2017 9,847 6 View

Bacterial cells grew with only gene of interest and no plasmid?

Hello, I did a ligation using pCDNA3.1 (5.4 kb) and a gene of interest(1.1kb). I transformed and plated DH5alpha cells with the following: pCDNA3.1+gene, pCDNA3.1 linearized, pCDNA3.1...

12 December 2016 1,665 4 View

What makes an antibody suitable for a specific assay?

I am currently working on immunoprecipitating a membrane receptor using a monoclonal antibody that has been validated for use in flow and immunohistochemistry. I understand polyclonal antibodies...

05 May 2016 9,515 5 View