I've never worked with proteins. Do you know any protocol for isolation of mitochondrial proteins from plant tissues?
Hello Sayed,
here you will find a lot of useful information on how to isolate mitochondria from plant tissues:
http://5e.plantphys.net/article.php?id=495
Best regards!
...
28 July 2024 6,080 0 View
Hi! I need to prepare Ti3C2Tx dispersion in water and through reviewing the relevant papers, I noticed that some researchers have reported Argon as the atmosphere for sonication while others...
28 December 2023 3,496 4 View
hey everybody need a hand in providing a few basic formula for "dynamic pricing"; maybe in airline industry or whatever; I would appreciate.
23 November 2023 3,805 2 View
Hello everyone, I'm conducting research on the influence of business model disclosure on external financing status and earnings persistence among companies listed on the New York Stock Exchange...
19 November 2023 9,364 3 View
Hello, I am making mesh on semi elliptical crack in Abaqus. The problem is that to make mesh perpendicular to crack front I have to make partition at the face of crack. I am following Neuman and...
08 November 2023 3,008 0 View
I have analyzed 80 different CFRP infill plate shear walls with different fiber orientation, span length and ply thickness under cyclic loading. all the CFRP infill plates consists of 2 CFRP...
03 November 2023 6,042 0 View
I have an array antenna with slant dual-polarized (-45&+45) elements. The array is linear in the Y direction and the antenna propagates in the Z direction. So YZ-plane is the E-plane and...
02 November 2023 5,700 0 View
Hi all I am studying a cationic cluster containing one molecule of methanol and one molecule of halobenzene, I am investigating a possible intra-cluster reaction leading to the formation of...
29 October 2023 6,171 6 View
Hi all, In an attempt to reproduce the simulations in https://www.nature.com/articles/srep08405#:~:text=However%2C%20in%20the%20case%20of,into%20a%20phosphorprotein%20interaction%20domain. I'm...
30 September 2023 4,664 3 View
Hello, I want to ask, is there any effect of Sea water environment on the Kinematic hardening parameters? Usually we do in Air R=-1 fatigue test and get the hysteresis loop. If we circulate 3.5%...
03 September 2023 4,455 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View