I have ITC Data needing interpretations. Can anyone help interpret such data?

10 November 2014 8,561 13 View

How valid are results from ITC with enzymes having multi-substrates?

My enzyme has 3 substrates. I want to determine Kd of each substrates using isothermal titration calorimetry. However, the first substrate does not "bind" with the enzyme unless the second...

07 August 2014 1,574 4 View

What is the next step after poly-alanine molecular replacement?

After a nightmare of MR trials, I've finally got a resonable match using poly-ala model by first pruning (gedit) then chainsaw. Any recommendations of a good site for the next steps would be...

06 July 2014 7,609 5 View

Does anyone know an assay for an enzyme inhibited by O2?

I am convinced that my enzyme is inhibited by atmospheric oxygen. One supporting evidence is that translation of this protein is only seen in hypoxic conditions. I want to support my hypothesis....

02 March 2014 2,557 4 View

Anyone know how to get a clean sample with SEC?

I have tried multiple buffers/conditions/parameters to get a clean sample from size exclusion chromatography SEC using sephadex 200 AKTA fPLC. Anyone have any suggestion how i get a pure protein...

01 February 2014 4,876 6 View

Does anyone know a robust assay?

I need a robust assay to determine enzymatic activity of my protein. The two small molecules are not UV-Vis unique. The two small chemical molecules are Naphthelene backbone and an isoprene tail....

01 February 2014 6,101 5 View

Can anyone help with 260 nm abs for membrane protein?

I purified extensively my membrane protein, ionic exchange, superdex 200, his-bind, etc. SDSPAGE, Bradford test, Western have all shown pure protein protein. This protein is not known to associate...

01 February 2014 1,304 7 View

Membrane Protein Storage conditions?

What glycerol concentration is appropriate for storage of membrane protein in -80? It seems that 10%-30% is causing aggregation after I store it for 10 days.

31 December 2013 10,009 4 View

Is it common that the expected MW of my membrane protein in SDS-PAGE is lower/higher than what is the theoretical size?

Much thanks.

31 December 2013 9,598 20 View

What is the best way to prevent membrane protein aggregation?

I'm expressing a membrane protein, and it seems to be prone to aggregating during purification. I have played with the pI-pH of buffer. What other option(s) are there?

31 December 2013 6,222 40 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View