15 Questions 36 Answers 0 Followers
Questions related from Joe S Matarlo
My data fit nicely in sequential model 6 sites. 1) does this mean there is 6 sites in the protein that the inhibitor can bind? 2) we do expect this protein to be a hexamer in solution (by...
11 November 2014 8,769 13 View
My enzyme has 3 substrates. I want to determine Kd of each substrates using isothermal titration calorimetry. However, the first substrate does not "bind" with the enzyme unless the second...
08 August 2014 1,648 4 View
After a nightmare of MR trials, I've finally got a resonable match using poly-ala model by first pruning (gedit) then chainsaw. Any recommendations of a good site for the next steps would be...
07 July 2014 7,762 5 View
I am convinced that my enzyme is inhibited by atmospheric oxygen. One supporting evidence is that translation of this protein is only seen in hypoxic conditions. I want to support my hypothesis....
03 March 2014 2,632 4 View
I need a robust assay to determine enzymatic activity of my protein. The two small molecules are not UV-Vis unique. The two small chemical molecules are Naphthelene backbone and an isoprene tail....
02 February 2014 6,187 5 View
I purified extensively my membrane protein, ionic exchange, superdex 200, his-bind, etc. SDSPAGE, Bradford test, Western have all shown pure protein protein. This protein is not known to associate...
02 February 2014 1,388 7 View
I have tried multiple buffers/conditions/parameters to get a clean sample from size exclusion chromatography SEC using sephadex 200 AKTA fPLC. Anyone have any suggestion how i get a pure protein...
02 February 2014 4,947 6 View
I'm expressing a membrane protein, and it seems to be prone to aggregating during purification. I have played with the pI-pH of buffer. What other option(s) are there?
01 January 2014 6,445 40 View
What glycerol concentration is appropriate for storage of membrane protein in -80? It seems that 10%-30% is causing aggregation after I store it for 10 days.
01 January 2014 10,115 4 View
Much thanks.
01 January 2014 9,790 20 View
Some bacteria have two enzymes that do the same thing, albeit one is much more efficient than the other and at the same time at higher endogenous concentration as well. Why would a bacteria would...
09 September 2013 1,753 8 View
SlyD seems to be a problem when im purifying my membrane protein. Why is it binding so tightly and how can I prevent this?
08 August 2013 9,423 6 View
Does anyone know a good starting point/generic Triton X100 (or any detergent) protocol?
04 April 2013 4,078 1 View
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03 March 2013 4,252 4 View
What is the most efficient way? And what plasmid do you use?
02 February 2013 7,406 2 View
