I have extracted gDNA from mononuclear cells using TRIZOL reagent, but it doesn't get dissolved in TE buffer. Why is that so? And is it possible to dissolve it in TE buffer?
Please kindly share your experience and method to dissolve extracted DNA in TE buffer. Thanks!
When you use Trizol to extract DNA, the final DNA pellet should be dissolved in a mild alkaline solution (8 mM NaOH). After full solubilization, adjust pH to desired one with HEPES free acid.
When you use Trizol to extract DNA, the final DNA pellet should be dissolved in a mild alkaline solution (8 mM NaOH). After full solubilization, adjust pH to desired one with HEPES free acid.
It could be due to over drying of pellet. After adding TE incubate in 37C for 10-15 min or till it dissolves(or a little bit longer: 60 min). Cheers, Nadine
I had decided to incubate my sample in 60C for 10 minutes, and it worked successfully. There was still a gooey clot remain but after I dissolve twice the volume and incubate it for another 10 minutes it turned out just fine and the yield was also quite high (around 500 ng/ul.) Thanks for all your answers! :)
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