Ariananda Hariadi

5 Questions 6 Answers 0 Followers

Questions related from Ariananda Hariadi

Is it possible to detect insertion or deletion mutation without cloning the target gene?

I am trying to detect mutation with sanger sequencing method. But some of my data have quite ugly electropherogram with the forward primer (sense strand) and better electropherogram on the other...

22 March 2015 2,464 3 View

Do we have to produce a single and specific band with electrophoresis before doing DHPLC?

Do we have to be sure that our PCR product produces single bands in electrophoresis before we get into DHPLC? Can you guys please recommend to me some books that will help me learn DHPLC for...

25 January 2015 9,603 3 View

How do you dissolve DNA pellet in TE Buffer?

I have extracted gDNA from mononuclear cells using TRIZOL reagent, but it doesn't get dissolved in TE buffer. Why is that so? And is it possible to dissolve it in TE buffer? Please kindly share...

23 October 2014 5,738 5 View

What is the optimal amount of RNA to do Reverse Transcription with RevertAid?

I did a cDNA synthesis using 500ng of RNA with helps of RevertAid enzyme from ThermoScientific. The result is quite surprising, because from the electrophoresis of PCR product, I concluded that...

15 October 2014 6,698 4 View

What is the best way to store DNA for long time between NaOH 8mM, nuclease free water, or TE buffer?

I would like to do DNA isolation by using trizol reagent and I am going to use it for PCR and DHPLC. What is the best way to store it? They say TE is not so good because it might intervene the...

11 October 2014 3,803 4 View