I would like to do DNA isolation by using trizol reagent and I am going to use it for PCR and DHPLC.
What is the best way to store it?
They say TE is not so good because it might intervene the Electrophoresis in DHPLC and PCR processes, but I am not sure that water is safe enough to store DNA for long time. What is your opinion? Thanks, in advance.
Hello Ariananda
I isolated DNA from PBMC by trizol or with QIAamp DNA. The DNA were stored in 8 mM NaOH or Buffer AE (10 mM Tris.CL; 0.5 mM EDTA; pH 9) at -80 °C respectively. DNA in water is subject to degradation by acid hidrolisis. I used DNA for HLA typing by final point PCR and were ok despite the DNA were store for more than 2 years.
I used 1X TE buffer to store my DNA at -80 °C and found the DNA apt for PCR analysis even after 4 years, so for me TE buffer is suitable. However, I don't whether it is the best way or not.
TE buffer works fine for me, didn't interfere with any PCR procedures that I did later.
One thing you must safeguard is frequent freeze thaws. Keep your samples in small aliquots and take out from the fridge only the amount you need at that time.
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