I want to know how to determine the exact ratio between N/P -chitosan/siRNA delivery for in vitro study.
First calculate the number of Nitrogen (N) in chitosan via ninhydrin test.
Calculate the number of phosphates in the siRNA based on number of basepairs. and find the ratio.
The supplementary section of following paper may give you better insight.
Article New Frontiers in Pest Control: Chitosan Nanoparticles-Shield...
Henry Kolge thank you
I'd like to see the output of every layer of DNN under OpenCV Python. So, I am asking you here to help me. If anyone knows a blog or something else explain how to show us outputs of layers,...
25 May 2020 222 9 View
I read more paper and wrote for example: "real-time detection of people from the RGB-D image" or "robust detection and identification of a person in real-time (around 0.3 s)"
04 May 2020 4,581 1 View
dispersion of hybrid nanofluid by use Cuo and Al2O3 nanoparticles in the water as a base fluid
22 December 2019 6,282 3 View
human following robot using deep learning and depth image
08 June 2019 7,749 6 View
[Synthesizing Cu nanoparticles without using chemical reductants]
01 March 2021 8,359 1 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...
15 February 2021 4,319 4 View
i am having a problem with the transfection of 2 siRNA , one for the longer isoform U and the other one of the sliced isoform S that is also the only transcribed. i am trasfection siRNA with a...
11 February 2021 8,145 3 View
Dear Researcher Respectfully, I am working on drug delivery. I synthesized Zn-MOF. I am going to load Doxorobicin on it and the study the derivery behaviour. I tried several times to load. but I...
07 February 2021 748 2 View
Currently, I am working on the PLA-PEG polymer micelle for the drug delivery system. The micelle I have synthesized I got avg 900nm particle size on DLS. I want to reduce the particle size below...
31 January 2021 2,684 5 View
hello I am working on a project that includes gene delivery to the brain. my intentions are encapsulating a gene in a Chitosan NP and IV administration the NP to rats. beside plasmids is there...
29 January 2021 4,455 5 View
I am working with low DD% chitosan. It doesn't dissolve completely in acidic solutions. How to calculate the degree of deacetylation by potentiometric and acid-base titration, UV spectroscopic...
27 January 2021 8,532 2 View
nanoparticles coated with chitosan as well uncoated nanoparticle
26 January 2021 9,053 6 View