How do you determine the ratio N/P in PEI/siRNA or Chitosan/siRNA delivery?

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Henry Kolge

First calculate the number of Nitrogen (N) in chitosan via ninhydrin test.

Calculate the number of phosphates in the siRNA based on number of basepairs. and find the ratio.

The supplementary section of following paper may give you better insight.

Article New Frontiers in Pest Control: Chitosan Nanoparticles-Shield...

Moqbel Ali Moqbel Redhwan

Henry Kolge thank you