How do you make experimental procedures to determine recovery in an analytical procedure?
How do you calculate it?
Please, put an example about your experimental procedures in case you use protein precipitation or SPE, LLE or another different procedures.
Hi Carlos!
As Angus said, spiking blank samples with three different concentrations works well. The concentrations chosen could be low, medium and high (depending upon the calibration points). The samples can then be analysed and quantified to determine the recovery.
If the method involves sample purification such as SPE, then a set of blank samples could be spiked with standards before the SPE extraction and a set of samples spiked after the SPE extraction. The latter is considered as 100% recovery. The peak area ratios between the analyte and internal standard of both sets can be compared.
Good luck and hope that's useful!
Please read: O. Gonzales et al. J. Chromatogr. A. 1353 (2014), 10-27, or
P.L.Cole et al. Biomed. Chromatogr. 25 (2011) 199-217, or
http://www.raps.org/regulatoryDetail.aspx?id=9422
Hi Carlos!
As Angus said, spiking blank samples with three different concentrations works well. The concentrations chosen could be low, medium and high (depending upon the calibration points). The samples can then be analysed and quantified to determine the recovery.
If the method involves sample purification such as SPE, then a set of blank samples could be spiked with standards before the SPE extraction and a set of samples spiked after the SPE extraction. The latter is considered as 100% recovery. The peak area ratios between the analyte and internal standard of both sets can be compared.
Good luck and hope that's useful!
I assessed five standard materials or more!!! The recoveries of target element were similar and proportional: Example: 80, 85, 83, 81, 82. Can I apply a correction factor for my samples???? The mean recovery was 82. So, can I use the factor 1.2??
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