I'd like to know how you guys detect mCherry in your transfected cells, e.g. in FACS, to check the efficiency or to sort. I mean, which wavelength are picked for excitation or which channels for detection?.
Dear Jahangir,
mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm. You can detect mCherry in the PE-TexasRed channel with a 610/20 bandpass filter.
Genemedi got a rich experience in virus production and transfection, you could find more information on https://www.genemedi.net/i/lentivirus-packaging
Hope this may help you.
Take a look here: https://www.chroma.com/spectra-viewer
You'll find the spectra of many fluorescent molecules, including mCherry
Dear Jahangir,
mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm. You can detect mCherry in the PE-TexasRed channel with a 610/20 bandpass filter.
Genemedi got a rich experience in virus production and transfection, you could find more information on https://www.genemedi.net/i/lentivirus-packaging
Hope this may help you.
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