Can anyone help with CD31 (PECAM-1) immunofluorescence protocol in PFA fixed tissues?

I am trying to use an anti-CD31, or PECAM-1, antibody to visualize vascularization in mouse bone marrow tissues. The antibody I'm using is MAB1398, clone 2H8, hamster monoclonal. It gives a very...

04 April 2014 5,134 31 View