06 June 2018 1 8K Report

I'd like to perform something like hierarchical clustering and then determine association/correlation with biologic signaling pathways

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How do you detect mCherry in lentivirus-transduced cell lines?

I'd like to know how you guys detect mCherry in your transfected cells, e.g. in FACS, to check the efficiency or to sort. I mean, which wavelength are picked for excitation or which channels for...

02 March 2015 990 3 View

How can I measure MiRNA in a tumor sample?

Hi all, I wonder how you measure miRNA in a sample retrieved from a tumor in a xenograft model. After tumor developed, miRNA was injected intratumorarly to look for effects on tumor growth. If you...

06 July 2014 2,072 7 View

How do you perform miRNA expression profiling in a co-culture system?

I wonder how you explore the pattern of miRNA or gene alterations in a "cell-cell contact" co-culture model (not a transwell). I mean how to know the changes come from the cell type you focus on.

04 May 2014 3,381 1 View

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