I'd like to perform something like hierarchical clustering and then determine association/correlation with biologic signaling pathways
R in combination with Bioconductor packages.
https://www.bioconductor.org/
I'd like to know how you guys detect mCherry in your transfected cells, e.g. in FACS, to check the efficiency or to sort. I mean, which wavelength are picked for excitation or which channels for...
02 March 2015 1,040 3 View
Hi all, I wonder how you measure miRNA in a sample retrieved from a tumor in a xenograft model. After tumor developed, miRNA was injected intratumorarly to look for effects on tumor growth. If you...
06 July 2014 2,109 7 View
I wonder how you explore the pattern of miRNA or gene alterations in a "cell-cell contact" co-culture model (not a transwell). I mean how to know the changes come from the cell type you focus on.
04 May 2014 3,427 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View